02. May 2018
One of the most common classes of excipients used in protein formulations are buffers. The aim of this work is to investigate the effect of buffers on protein stabilization given by sugars during freeze drying. Methods Molecular Dynamics simulations of human growth hormone (hGH) in the presence of sucrose and trehalose were performed, and the impact of phosphate and citrate buffers on their protective action was analyzed. Results We found that buffers broke the hydrogen bonding network formed...
16. April 2018
Surfactants are common stabilizers, often added to biopharmaceuticals formulations, but the mechanisms at the basis of their activity are unclear. The aim of this work is to provide insight into the molecular factors underlying surfactant effectiveness as protectants. Methods Molecular Dynamics simulations of human growth hormone (hGH) in the presence of Tween 20 were performed. The effect of Tween 20 was compared with the activity of commonly used protectants, such as the disaccharides....
09. April 2018
Some impressions of the 2018 PBP Worldmeeting on Pharmaceutics, Biopharmaceutics and Pharmaceutical Technology in Granada with over 1.300 participants. 4 parallel session on hot topics, poster session and accompanying Exhibition by ResearchPharm allowed intense exchange amongst the participipants. More Information on the event
24. April 2017
Abstract Reducing the promiscuous tropism of native adenovirus by using fiberless adenovirus is advantageous towards its use as a gene therapy vector or vaccine component. The removal of the fiber protein on native adenovirus abrogates several undesirable interactions; however, this approach decreases the particle’s physical stability. In order to create stable fiberless adenovirus for pharmaceutical use, the effects of temperature and pH on the particle’s stability profile must be...